Faculty Directory
Keith E.J. Tyo

Associate Professor of Chemical and Biological Engineering


2145 Sheridan Road
Tech E156
Evanston, IL 60208-3109

847-467-2972Email Keith Tyo


Tyo Research Group


Center for Synthetic Biology


Chemical and Biological Engineering


PhD Program in Interdisciplinary Biological Sciences

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Ph.D., MIT, Cambridge, MA

M.S., MIT, Cambridge, MA

B.S., West Virginia University, Morgantown, WV


Research Interests

Synthetic biology, metabolic engineering, global health

What we do
Microbes must cope with harsh, rapidly changing environments to survive. To do this, microbes have developed sophisticated mechanisms to (a) sense the changes in the environment, and (b) respond quickly to these changes to protect itself from harm or capitalize on an opportunity. Our lab seeks to rewire these fundamental input/output relationships to program cells to do useful things for mankind in a paradigm called synthetic biology. Inputs: We study methods to modify existing environmental detection sensors in yeast and modify them to detect new analytes. Outputs: We investigate ways microbes modify their metabolic networks and use these modifications to increase production of a given metabolite.

Why we do it
Synthetic biology offers a disruptive technology that has vast potential to impact the most important facets of our lives. The way we diagnose diseases, the drugs we use, the fuels we put in our cars, the plastic that is used in our potato chip bag - we have the opportunity to improve the sustainability of our lifestyle. And by reducing costs, we can make aspects of our lifestyle available to those who could not otherwise afford it. We hope to offer technical contributions that, simply put, make the world a better place.

Selected Publications

  • Jennifer Greene, James Daniell, Michael Kpke, Linda J Broadbelt, Keith Edward Jaggard Tyo, “Kinetic ensemble model of gas fermenting Clostridium autoethanogenum for improved ethanol production”, Biochemical Engineering Journal, (2019)
  • Aaron M. Love, Bradley W. Biggs, Keith Edward Jaggard Tyo, Parayil Kumaran Ajikumar, “Chemically inducible chromosomal evolution (CIChE) for multicopy metabolic pathway engineering”, Methods in Molecular Biology, (2019)
  • James Jeffryes, Jonathan Strutz, Chris Henry, Keith Edward Jaggard Tyo, “Metabolic in silico network expansions to predict and exploit enzyme promiscuity”, Methods in Molecular Biology, (2019)
  • Adebola Adeniran, Sarah Stainbrook, John W. Bostick, Keith Edward Jaggard Tyo, “Detection of a Peptide Biomarker by Engineered Yeast Receptors”, ACS Synthetic Biology, (2018)
  • Alexandra M. de Paz, Thaddeus R. Cybulski, Adam H. Marblestone, Bradley M. Zamft, George M. Church, Edward S. Boyden, Konrad P. Kording, Keith Edward Jaggard Tyo, “High-resolution mapping of DNA polymerase fidelity using nucleotide imbalances and next-generation sequencing”, Nucleic Acids Research, (2018)
  • Andrew K.D. Younger, Peter Y. Su, Andrea J. Shepard, Shreya V. Udani, Thaddeus R. Cybulski, Keith Edward Jaggard Tyo, Joshua N Leonard, “Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion”, Protein Engineering, Design and Selection, (2018)
  • Li Wang, Chenchen Liang, Jing Wu, Liming Liu, Keith Edward Jaggard Tyo, “Increased processivity, misincorporation, and nucleotide incorporation efficiency in Sulfolobus solfataricus Dpo4 thumb domain mutants”, Applied and Environmental Microbiology, (2017)
  • Jennifer L. Greene, Andreas Waechter, Keith Edward Jaggard Tyo, Linda J Broadbelt, “Acceleration Strategies to Enhance Metabolic Ensemble Modeling Performance”, Biophysical Journal, (2017)